Tag: western blot
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Common Buffer Recipes for Research Lab
In bio labs, it’s often necessary to prepare common buffers for various experiments. I’ve decided to create this post as a memo for myself as a handy reference. Over time, I’ll be updating this post with buffer recipes (often available on the Internet somewhere) with helpful tips and tricks for their preparation. Gelatin Zymography Gelatin…
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Studying Enzyme Activity with Zymography
In a previous post, I talked about immunostaining and touched on the principles and procedures of Western blotting, a technique that is widely used to quantify protein expression. Now, let’s turn our attention to another powerful molecular tool that is often employed in research laboratories: zymography. Distinct from Western blotting, this technique allows for the…
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Quantifying Protein with Western Blot
Recently, I’ve been working as a research assistant in a lab and finally came across the technique of Western blot. I didn’t have any training on this technique because I didn’t take molecular biology lab in my undergraduate career, so I had to learn it on the spot. I decided to write this blog post…