Category: Research
-
Common Buffer Recipes for Research Lab
In bio labs, it’s often necessary to prepare common buffers for various experiments. I’ve decided to create this post as a memo for myself as a handy reference. Over time, I’ll be updating this post with buffer recipes (often available on the Internet somewhere) with helpful tips and tricks for their preparation. Gelatin Zymography Gelatin…
-
Useful Numbers for Various Cell Culture Dishes
There are many different sizes of dishes and flasks that we use for cell culture in our lab. It’s very important to know the surface area and volumes of media or reagents to add. Since we are using the Nunc™ Cell-Culture plates, I copied and pasted some useful numbers from ThermoFisher website.
-
Studying Enzyme Activity with Zymography
In a previous post, I talked about immunostaining and touched on the principles and procedures of Western blotting, a technique that is widely used to quantify protein expression. Now, let’s turn our attention to another powerful molecular tool that is often employed in research laboratories: zymography. Distinct from Western blotting, this technique allows for the…
-
Quantifying Protein with Western Blot
Recently, I’ve been working as a research assistant in a lab and finally came across the technique of Western blot. I didn’t have any training on this technique because I didn’t take molecular biology lab in my undergraduate career, so I had to learn it on the spot. I decided to write this blog post…
-
Colony PCR: A Powerful Tool for Yeast Analysis
Yeast colony PCR is a variation of the colony PCR technique that is used to amplify DNA from a single yeast colony. Like regular colony PCR, yeast colony PCR involves transferring a single colony of yeast to a PCR tube, adding PCR buffer, primers, and other reagents, and then running the reaction in a thermal…