There are many different sizes of dishes and flasks that we use for cell culture in our lab. It’s very important to know the surface area and volumes of media or reagents to add. Since we are using the Nunc™ Cell-Culture plates, I copied and pasted some useful numbers from ThermoFisher website.

	Surface area (cm2)	Seeding density*	Cells at confluency*	Trypsin (mL of 0.05% trypsin, 0.53 mM EDTA).	Growth medium (mL)
Dishes
35mm	8.8	0.3 x 106	1.2 x 106	1	2
60mm	21.5	0.8 x 106	3.2 x 106	3	5
100mm	56.7	2.2 x 106	8.8 x 106	5	12
150mm	145	5.0 x 106	20.0 x 106	10	30
Culture plates
6-well	9.6	0.3 x 106	1.2 x 106	1	1 to 3
12-well	3.5	0.1 x 106	0.5 x 106	0.4 to 1	1 to 2
24-well	1.9	0.05 x 106	0.24 x 106	0.2 to 0.3	0.5 to 1.0
48-well	1.1	0.03 x 106	0.12 x 106	0.1 to 0.2	0.2 to 0.4
96-well	0.32	0.01 106	0.04 x 106	0.05 to 0.1	0.1 to 0.2